Journal: Anatomical record (Hoboken, N.J. : 2007)
Article Title: Maternal hypoxia developmentally programs low podocyte endowment in male, but not female offspring.
doi: 10.1002/ar.24369
Figure Lengend Snippet: FIGURE 1 Confocal microscopy of four whole glomeruli at postnatal Day 21 in male normoxic (a) and male hypoxic (b), female normoxic (c), and female hypoxic (d) mice. P57 immunofluorescence (red) indicates podocyte nuclei. Synaptopodin (green) immunostaining indicates podocyte cytoplasm
Article Snippet: One midhilar slice per kidney then underwent 1 hr of antigen retrieval in Dako Citrate Antigen Retrieval Solution (S1699; Dako, Glostrup, Denmark) on a Dako PT Link Instrument (PT10126; Dako) for 60 min at 98 C. Slices were then incubated for 6 days on an orbital shaker (OM8, Ratek, Australia) at 37 C with two primary antibody solutions for podocyte labeling, namely polyclonal rabbit anti-mouse p57 antibody (1:200, Santa Cruz Biotechnology, Santa Cruz, CA; sc-8,298; for labeling of podocyte nuclei) and polyclonal goat anti-mouse synaptopodin (1:400, Santa Cruz Biotechnology, Santa Cruz, CA; sc-21537; for labeling of podocyte cytoplasm).
Techniques: Confocal Microscopy, Immunofluorescence, Immunostaining